For in vitro laboratory research only. Not for human or veterinary consumption.
China Warehouse PNC-27 10mg | 10-Vial Kit — p53-derived proapoptotic peptide — penetrates HDM-2-overexpressing cancer cell membranes to trigger membranolytic apoptosis selectively in tumour cells that overexpress HDM-2. ≥99% HPLC purity, MS confirmed, Janoshik COA. Research use only. International shipping: 7–14 days
PNC-27 is a 32-amino acid chimeric anticancer peptide containing a p53 HDM-2-binding domain fused to a membrane-destabilising penetratin sequence. It binds HDM-2 (human MDM-2) expressed on the plasma membrane of cancer cells — an expression pattern absent in normal cells — inducing selective membranolysis of tumour cells via a pore-forming mechanism. This selectivity makes PNC-27 a highly specific research tool for studying cancer cell membrane disruption and MDM-2-targeted therapy without affecting normal cell viability.
Research Applications
Selective Tumour Cell Lysis Research
PNC-27 lyses cancer cell lines expressing plasma membrane HDM-2 while sparing normal cells. Standard in vitro readouts: LDH release assay, PI exclusion, MTT viability across cancer vs normal cell panels.
MDM-2/p53 Pathway Research
PNC-27 exploits cancer-specific plasma membrane HDM-2 expression — distinct from nuclear MDM-2 p53 degradation. Research into membranous MDM-2 as a cancer-specific target.
Cancer Cell Line Selectivity Profiling
Mapping HDM-2 plasma membrane expression across cancer cell lines to identify PNC-27-responsive tumour types — pancreatic, breast, and melanoma lines show high sensitivity.
Add 1–2mL bacteriostatic water to the lyophilized vial by directing water down the inner wall of the vial. Swirl gently until the powder fully dissolves — do not shake or vortex. The solution should be clear and colourless. Use immediately or refrigerate at 2–8°C.
How should this peptide be stored?
Store lyophilized peptide vials at −20°C for long-term storage. Once reconstituted, store at 2–8°C (refrigerator) and use within 21–28 days. Avoid repeated freeze-thaw cycles, direct light, and heat exposure.
What purity is this Vesta compound and how is it verified?
All Vesta compounds are tested to ≥99% purity by HPLC peak area analysis on every production batch. Molecular identity is confirmed by mass spectrometry. A Certificate of Analysis (COA) is published on the product page and independently verifiable at verify.janoshik.com using the batch reference number.
Peptide Research Tools: Use Vesta’s free peptide calculators — reconstitution, BAC water, dosing, half-life, and mixing charts.
International shipping: 7–14 days
Research use only
This material is supplied strictly for in vitro laboratory research. It is not intended for human or veterinary consumption, diagnosis, treatment, or household use.
